Identification and characterization of the pckA gene from Staphylococcus aureus.
نویسندگان
چکیده
The Staphylococcus aureus pckA gene was identified and characterized. A pckA mutant lacked detectable phosphoenolpyruvate carboxykinase activity and grew poorly in the absence of glucose. Both enzymatic activity and pckA promoter activity in wild-type cells grown in the absence of glucose were at least 22-fold greater than activities in cells grown in the presence of glucose.
منابع مشابه
Identification and Characterization of a High Vancomycin-Resistant Staphylococcus aureus Harboring VanA Gene Cluster Isolated from Diabetic Foot Ulcer
Objective(s) Staphylococcus aureus is a common cause of human infection, and emergence of vancomycin-resistance S. aureus is a great concern for treatment of methicillin-resistant S. aureus,(MRSA) in recent years (MRSA). Here, we report the isolation of high-level VRSA. Materials and Methods S. aureus was isolated from foot ulcer of a diabetic woman in Tehran, Iran. Antibiotic susceptibilit...
متن کاملIdentification of Toxic Shock Syndrome Toxin-1 (TSST-1) gene in Staphylococcus aureus isolated from bovine mastitis milk
Staphylococcus aureus is a major causative pathogen of clinical and subclinical mastitis of dairy domestic ruminants. This agent produces a variety of extracellular toxins and virulence factors including Toxic Shock Syndrome Toxin-1 (TSST-1) which is the major cause of Toxic Shock Syndrome (TSS). In this study 58 S. aureus isolates obtained from 9 dairy herds in East and West Azerbaijan provinc...
متن کاملPCR-mediated identification of Methicillin and Vancomycin resistant genes in Staphylococcus aureus strains isolated from the nasal cavity
Staphylococcus aureus is colonized in the human nasal cavity and would contaminate hospital and therapeutic environments. This bacterium has a genetic diversity in terms of resistance to antimicrobial agents. Therefore, the purpose of this study was identificatied of Methicillin and Vancomycin resistant genes in Staphylococcus aureus strains which has been isolated from the nasal cavity. 189 pa...
متن کاملMolecular Characterization and SCCmec in Methicillin Resistant Staphylococcus aureus from Healthy Worker Nasal Swab in Larestan Hospital
Background and Aims: The aim of present study was to investigate the molecular characteristic of Staphylococcus aureus to detect mec A gene and to type SCCmec in strain isolated from healthy worker at Larestan Hospital. Material and Methods: This study was carried out from the 250 nasal swab healthy worker at Larestan hospital. Multiplex polymerase chain reaction for mecA gene was perf...
متن کاملIdentification of Virulence Genes in Staphylococcus aureus Isolates Segregated from Children’s Wounds
Background: Staphylococcus aureus is an opportunistic pathogenic bacterium that can provide invasive conditions by secreting various toxins. It is involved in the development of skin infections, such as swollen and ulcers. Today, one of the main problems is the treatment of Staphylococcus aureus resistant strains. The aim of the current study was to detect virulence factors and antibiotic re...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 178 11 شماره
صفحات -
تاریخ انتشار 1996